AA8細(xì)胞是CHO細(xì)胞的衍生株，含有21條染色體，并且腺嘌呤磷酸核糖轉(zhuǎn)移酶（APRT）位點(diǎn)雜合。該細(xì)胞常用于基因突變檢測，是一系列修復(fù)缺失突變細(xì)胞株（如UV敏感突變株UV5、UV20、UV41，DNA修復(fù)突變株EM9）的親本細(xì)胞。經(jīng)無支原體鑒定入庫。

動(dòng)物種別：中國倉鼠

組織來源：卵巢

形態(tài)：上皮細(xì)胞樣，單層和懸浮生長

培養(yǎng)基配方：

90%MEM(MD0300)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培養(yǎng)基：MEM完全培養(yǎng)基（MD0301）

氣相：空氣，95%；二氧化碳，5%。 溫度：37℃。

凍存條件：基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS(SD0200)

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

聲明：產(chǎn)品僅科研使用。

PubMed=7360155; DOI=10.1016/0165-1161(80)90188-0

Thompson L.H., Fong S., Brookman K.

Validation of conditions for efficient detection of HPRT and APRT mutations in suspension-cultured Chinese hamster ovary cells.

Mutat. Res. 74:21-36(1980)

PubMed=7404270; DOI=10.1007/BF01542791

Thompson L.H., Rubin J.S., Cleaver J.E., Whitmore G.F., Brookman K.

A screening method for isolating DNA repair-deficient mutants of CHO cells.

Somatic Cell Genet. 6:391-405(1980)

PubMed=3347204; DOI=10.1016/0167-8817(88)90041-7

Fuller L.F., Painter R.B.

A Chinese hamster ovary cell line hypersensitive to ionizing radiation and deficient in repair replication.

Mutat. Res. 193:109-121(1988)

PubMed=2687628; DOI=10.1093/mutage/4.5.349

Busch D.B., Greiner C., Lewis K., Ford R., Adair G.M., Thompson L.H.

Summary of complementation groups of UV-sensitive CHO cell mutants isolated by large-scale screening.

Mutagenesis 4:349-354(1989)

PubMed=2218721; DOI=10.1007/BF01232460

Wahls W.P., Moore P.D.

Relative frequencies of homologous recombination between plasmids introduced into DNA repair-deficient and other mammalian somatic cell lines.

Somat. Cell Mol. Genet. 16:321-329(1990)

PubMed=8765433; DOI=10.1007/s002800050504

Andersson B.S., Sadeghi T., Siciliano M.J., Legerski R., Murray D.

Nucleotide excision repair genes as determinants of cellular sensitivity to cyclophosphamide analogs.

Cancer Chemother. Pharmacol. 38:406-416(1996)

DOI=10.3390/pr1030296

Wurm F.M.

CHO quasispecies -- implications for manufacturing processes.

Processes 1:296-311(2013)