這是一款，通過直腸穿刺活檢(TRBP)建立自體永生化前列腺癌細胞系，該穿刺活檢前列腺癌(CRPC)患者。Bob是CRPC功能研究，特別是CRPC“基礎”表型研究的一種新的臨床前模型。已知的現象是，由于癌細胞系的遺傳不穩(wěn)定性增加，Y染色體可能會重新排列或丟失，導致缺乏檢測。根據STR-PCR分析，該細胞系與存款人提供的來源相同。

生長特性：鵝卵石樣，貼壁生長

培養(yǎng)基：前列腺上皮細胞培養(yǎng)基

Keratinocyte-SFM supplied with prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53), bovine pituitary extract (BPE) and glutamine) + 2ng/ml leukaemia inhibitory factor, 2ng/ml stem cell factor + 100ng/ml cholera toxin + 1ng/ml granulocyte macrophage colony stimulating factor.

培養(yǎng)和凍存方式：

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells must be centrifuged at 400 x g for 5 minutes to remove the trypsin at each subculture. Cells are cryopreserved in 45% conditioned media: 45% fresh media: 10% DMSO. At confluence 5-7 x 104 cells/cm2 can be expected.

說明：產品僅供科研使用