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產(chǎn)品描述/Products Description
E006AA是一種自發(fā)的永生化細(xì)胞系，來源于一名非裔美國(guó)人后裔體內(nèi)的Gleason 6局限性前列腺癌。這種細(xì)胞系異種移植到完整或閹割的雄性NOG-SCID小鼠體內(nèi)生長(zhǎng)同樣良好，且在裸鼠中無致瘤性。E006AA應(yīng)與E006AA-ht并行用于前列腺癌早期建模。
培養(yǎng)基：前列腺上皮細(xì)胞培養(yǎng)基
Keratinocyte-SFM supplied with prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53), bovine pituitary extract (BPE) and glutamine) + 2ng/ml leukaemia inhibitory factor, 2ng/ml stem cell factor + 100ng/ml cholera toxin + 1ng/ml granulocyte macrophage colony stimulating factor.
培養(yǎng)和凍存方式：
Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells must be centrifuged at 400 x g for 5 minutes to remove the trypsin at each subculture. Cells are cryopreserved in 45% conditioned media: 45% fresh media: 10% DMSO. At confluence 5-7 x 104 cells/cm2 can be expected.
說明：產(chǎn)品僅供科研使用
