背景介紹/context：

16HBE14o- is a human bronchial epithelial cell line isolated from a 1-year old male heart-lung patient and immortalized with the origin-of-replication defective SV40 plasmid (pSVori-). The cell line retains characteristic features of normal differentiated bronchial epithelial cells including a cobblestone morphology, cytokeratin expression, the ability to form tight junctions, and directional ion transport. When grown with an air/liquid interface, cilia can be detected. In contrast to most other respiratory cell lines, 16HBE14o- expresses high levels of cystic fibrosis transmembrane conductance regulator (CFTR) mRNA and protein. Expression of CFTR is correlated to cAMP-dependent Cl- conductance in a variety of cells, including 16HE14o- epithelial cells.

16HBE14o-是從一名1歲男性心肺患者身上分離出來的人支氣管上皮細胞系，具有復制起源缺陷SV40質粒(pSVori-)。該細胞系保留了正常分化支氣管上皮細胞的特征，包括鵝卵石狀的形態(tài)，細胞角蛋白的表達，形成緊密連接的能力，以及定向離子運輸。當生長在一個空氣/液體的界面，纖毛可以被檢測。與大多數(shù)其他呼吸細胞系相比，16HBE14o-表達高水平的囊性纖維化跨膜電導調節(jié)(CFTR) mRNA和蛋白。在包括16HE14o上皮細胞在內的多種細胞中，CFTR的表達與camp依賴的Cl-電導相關。

動物種別/Organism  人

組織來源 Tissue and Cell Type 支氣管上皮細胞

形態(tài)/Morphology 上皮細胞樣，貼壁生長 

注意事項：此產(chǎn)品僅供科研使用