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攜帶GFP-hTert基因的人永生化視網(wǎng)膜微血管周細(xì)胞
貨號(hào)：IM037
價(jià)格： ￥詢價(jià)
產(chǎn)品描述/Products Description
背景介紹/context：
HRMVPCs are initiated by elutriation from dissociated normal human retinal tissue. Puromycin resistant GFP-hTert-HRMVPCs are selected from HRMVPCs after infected with lentiviruses expressing GFP and hTert. The cells are shipped in frozen vials (the cells are provided @ passage 4). Pericyte Growth Medium is recommended for cell culture and these cells have a minimum of population doubling capacity > 30 (tested so far) when cultured following the detailed protocol described below.
HRMVPCs是由分離的正常人視網(wǎng)膜組織的淘洗啟動(dòng)的。從HRMVPCs感染表達(dá)GFP和hTert的慢病毒后，選擇具有普羅霉素抗性的GFP-hTert-HRMVPCs。細(xì)胞用冷凍小瓶運(yùn)輸(細(xì)胞見(jiàn)第4篇)。推薦周細(xì)胞生長(zhǎng)培養(yǎng)基進(jìn)行細(xì)胞培養(yǎng)，這些細(xì)胞至少具有增殖能力倍增的能力。按照以下詳細(xì)的培養(yǎng)方案培養(yǎng)30例(目前已測(cè)試)。
動(dòng)物種別/Organism 人
組織來(lái)源 Tissue and Cell Type 視網(wǎng)膜組織
形態(tài)/Morphology 貼壁生長(zhǎng)
注意事項(xiàng)：此產(chǎn)品僅供科研使用
