背景介紹/context：

IDG-SW3 represent a non-homogenous population progressing from early osteoblasts to late osteocytic. These cells express functional SV40 large T antigen that is induced in the presence of IFN γ under permissive temperature (33°C) and express GFP under control of the dentin matrix (Dmp1) promoter. Initial culture shows osteoblastic phenotype, however when under mineralizing conditions, the cells start to express early osteocyte markers such as E11/podoplanin, followed by Dmp1 and mature markers SOST and FGF23 by 21-28 days of culture. 

Similar to osteocytes in vivo, these cells respond to hormonal signals such as PTH and 1,25-dihydroxyvitamin-D3. IDG-SW3 cells can be maintained in both 2D and 3D cultures and are useful for study of osteocytes at various differentiation stages. It should be noted that these cultures may contain a mixture of osteoblasts and osteocytes at different stages of differentiation and for studies requiring enriched osteocytes, the Dmp1-GFP cells should be isolated by flow cytometry (FACS).

IDG-SW3代表一個從早期成骨細胞發(fā)展到晚期成骨細胞的非同質(zhì)群體。這些細胞表達功能SV40大T抗原，該抗原是在允許溫度下(33 C) IFN不表達的情況下誘導產(chǎn)生的，并在牙本質(zhì)基質(zhì)(Dmp1)啟動子的控制下表達GFP。初始培養(yǎng)表現(xiàn)成骨細胞表型，但在礦化條件下，細胞開始表達早期骨細胞標志物，如E11/podoplanin，培養(yǎng)21-28天后表達Dmp1和成熟標志物SOST和FGF23。與體內(nèi)骨細胞相似，這些細胞對激素信號如PTH和1,25-二羥維生素d3做出反應。IDG-SW3細胞可以在2D和3D培養(yǎng)中維持，對研究不同分化階段的骨細胞很有用。值得注意的是，這些培養(yǎng)物可能包含不同分化階段的成骨細胞和骨細胞的混合物，對于需要豐富骨細胞的研究，Dmp1-GFP細胞應該通過流式細胞術(shù)(FACS)分離出來。

動物種別/Organism  小鼠

組織來源 Tissue and Cell Type 成骨細胞

形態(tài)/Morphology 貼壁生長 

注意事項：此產(chǎn)品僅供科研使用