背景介紹/context：

Immortalised Mouse Kupffer Cells (ImKC) are isolated from male H-2Kb-tsA58 transgenic mice which expresses the thermolabile mutant tsA58 of SV40 40 large T antigen and were sorted through identifying the F4/80 marker. The Immortalised cells expresses p53 levels with increases telomerase activity and retains the some functional features compared to the primary cells. ImKC cells can propagate without the presence of IFNy at 37°C, and are able to produce proinflammatory cytokines when induced with lipopolysaccharide (LPS). These cells are valuable for studies in proinflammatory cytokines (TNF-α, IL-6 and IL-1β) and their roles in Kuppfer cells, in processes in liver injury, and for research in chronic diseases.

從表達(dá)SV40 40大T抗原的熱穩(wěn)定性突變體tsA58的雄性H-2Kb-tsA58轉(zhuǎn)基因小鼠中分離得到ImKC細(xì)胞，并通過鑒定F4/80標(biāo)記進(jìn)行分類。與原發(fā)細(xì)胞相比，永生細(xì)胞表達(dá)p53水平，端粒酶活性增加，并保留了一些功能特征。在沒有IFNy的情況下，ImKC細(xì)胞在37c可以增殖，在脂多糖(lipopolysaccharide, LPS)的誘導(dǎo)下可以產(chǎn)生促炎細(xì)胞因子。這些細(xì)胞對(duì)于研究促炎細(xì)胞因子(TNF- TNF、IL-6和IL-1細(xì)胞因子)及其在Kuppfer細(xì)胞中的作用、在肝損傷過程中的作用以及慢性疾病的研究都有價(jià)值。

動(dòng)物種別/Organism  小鼠

組織來源 Tissue and Cell Type Kuppfer細(xì)胞

形態(tài)/Morphology 貼壁生長(zhǎng) 

注意事項(xiàng)：此產(chǎn)品僅供科研使用