背景介紹/context：

The R28 cells were developed from E1A-NR.3 parental cell line through three rounds of limiting dilution and were therefore derived from a single cell. Despite their clonal origin, these cells display both glial and neuronal cell markers indicative of a retinal precursor cell.The parental line E1A-NR.3 was established by immortalization of postnatal day 6 rat neuroretinal tissue using the psi2 replication incompetent retroviral vector. As a result, these cells are already resistant to geneticin/G418 and would require an alternative selection marker for transfection studies. These cells were designed not to form tumors in animals.R28細胞來源于E1A-NR.3親本細胞系經(jīng)過三輪限制稀釋，因此從單個細胞中提取。盡管它們的起源是克隆的，這些細胞同時顯示膠質(zhì)細胞和神經(jīng)元細胞標記，指示視網(wǎng)膜前體細胞。母系E1A-NR。采用psi2復制不合格逆轉(zhuǎn)錄病毒載體對出生后6天大鼠神經(jīng)視網(wǎng)膜組織進行永生化。因此，這些細胞已經(jīng)對geneticin/G418具有抗性，需要另一種選擇標記來進行轉(zhuǎn)染研究。這些細胞被設(shè)計成不會在動物體內(nèi)形成腫瘤。

動物種別/Organism  大鼠

組織來源 Tissue and Cell Type 視網(wǎng)膜細胞

形態(tài)/Morphology 神經(jīng)細胞樣，貼壁生長 

注意事項：此產(chǎn)品僅供科研使用