背景介紹/context：

Each Retinal Cell Line (R28) Cytospin contains approximately 500-1,000 cells fixed to a microscope slide. These convenient cytospins can immediately be used for experiments instead of taking the time to grow the actual R28 cells (e.g. to quickly and easily check and see if the cells have a particular gene of interest). The cytospins are compatible with standard immunocytochemistry techniques or in situ hybridization.The R28 cells were developed from E1A-NR.3 parental cell line through three rounds of limiting dilution and were therefore derived from a single cell. Despite their clonal origin, these cells display both glial and neuronal cell markers indicative of a retinal precursor cell. The parental line E1A-NR.3 was established by immortalization of postnatal day 6 rat neuroretinal tissue using the psi2 replication incompetent retroviral vector. As a result, these cells are already resistant to geneticin/G418 and would require an alternative selection marker for transfection studies. These cells were designed not to form tumors in animals.

每個(gè)視網(wǎng)膜細(xì)胞系(R28)細(xì)胞涂片包含大約500- 1000個(gè)細(xì)胞固定在顯微鏡載玻片上。這些方便的細(xì)胞固定器可以立即用于實(shí)驗(yàn)，而不是花時(shí)間培養(yǎng)實(shí)際的R28細(xì)胞(例如，快速和容易地檢查和查看細(xì)胞是否有一個(gè)特定的基因感興趣)。這些胞質(zhì)酶與標(biāo)準(zhǔn)的免疫細(xì)胞化學(xué)技術(shù)或原位雜交技術(shù)兼容。R28細(xì)胞來(lái)源于E1A-NR。3親本細(xì)胞系經(jīng)過三輪限制稀釋，因此從單個(gè)細(xì)胞中提取。盡管它們的起源是克隆的，這些細(xì)胞同時(shí)顯示膠質(zhì)細(xì)胞和神經(jīng)元細(xì)胞標(biāo)記，指示視網(wǎng)膜前體細(xì)胞。母系E1A-NR。采用psi2復(fù)制不合格逆轉(zhuǎn)錄病毒載體對(duì)出生后6天大鼠神經(jīng)視網(wǎng)膜組織進(jìn)行永生化。因此，這些細(xì)胞已經(jīng)對(duì)geneticin/G418具有抗性，需要另一種選擇標(biāo)記來(lái)進(jìn)行轉(zhuǎn)染研究。這些細(xì)胞被設(shè)計(jì)成不會(huì)在動(dòng)物體內(nèi)形成腫瘤。

動(dòng)物種別/Organism  大鼠

組織來(lái)源 Tissue and Cell Type 視網(wǎng)膜細(xì)胞

形態(tài)/Morphology 貼壁生長(zhǎng) 

注意事項(xiàng)：此產(chǎn)品僅供科研使用