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產(chǎn)品描述/Products Description
背景介紹/context:
Human iPS (induced pluripotent stem) cell line was derived from human foreskin fibroblasts (HFFs) by retroviral expression of OCT4, SOX2, KLF4, and c-MYC genes. The cells were derived using morphological selection criteria and without the use of fluorescent marker or drug selection. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells is identical to that of human ES cells. The cells also express the pluripotency markers SSEA-3 and
Nanog, and demonstrate strong endogenous alkaline phosphatase activity. The low passage iPS cells are highly viable and have been pre-adapted to serum-free,
feeder-free culture conditions.
人iPS (induced pluripotent stem, induced iPS)細胞系來源于人包皮成纖維細胞(Human包皮成纖維細胞,HFFs),通過逆轉錄病毒表達OCT4、SOX2、KLF4和c-MYC基因。細胞的來源使用形態(tài)學選擇標準,沒有使用熒光標記或藥物選擇。在標準的人類胚胎干細胞培養(yǎng)條件下培養(yǎng),人類iPS細胞的形態(tài)與人類胚胎干細胞相同。這些細胞還表達了多能性標記SSEA-3和Nanog,并表現(xiàn)出很強的內源性堿性磷酸酶活性。低傳代誘導多能干細胞具有很高的存活能力,并且已經(jīng)預先適應了無血清、無刺激的培養(yǎng)條件。
動物種別/Organism 人
組織來源 Tissue and Cell Type iPS細胞
形態(tài)/Morphology 貼壁生長
注意事項:此產(chǎn)品僅供科研使用






