背景介紹/context：

It is well-established that induced pluripotent stem cells may be generated by co-transfection of four genes: Oct3/4, Sox2, Kfl4 and c-Myc, into somatic adult cells resulting in re-programming of these cells to pluripotency equivalent to that of embryonic stem cells. It has recently been shown by independent research including results from our laboratory that over-expression of a single gene, Oct3/4, in adult neural or mesenchymal stem cells also results in enhanced differentiation capacity including pluripotency. This development simplifies iPS generation and may reflect levels of endogenous expression of Sox2, Kfl4 and c-Myc in adult stem cells that are multipotent.

Also, since the Oct3/4 promoter has well-known response elements and transcription control mechanisms, re-programming based on environmental cues is now feasible through use of small molecular agents that selectively activate expression of Oct3/4. Our human MSCs expressing Oct3/4 allow further investigation of iPSC mechanisms.

Optimizing your hMSC Cultures: 

1) Grow cells in 1 to 5% O2, 5% CO2, with the balance of the gas content as N2..

2) Use of our MSCGroTM media is highly recommended.

3) Subtrate- We recommend Greiner Bio-one TC-treated cell culture flasks or equivalent.

誘導多能干細胞的產(chǎn)生是通過共轉(zhuǎn)染四種基因:Oct3/4、Sox2、Kfl4和c-Myc進入成體細胞，導致這些細胞重新編程，使其具有等同于胚胎干細胞的多能性。最近，包括我們實驗室結(jié)果在內(nèi)的獨立研究表明，在成人神經(jīng)或間充質(zhì)干細胞中，單個基因Oct3/4的過度表達也會導致分化能力的增強，包括多能性。這一進展簡化了iPS的產(chǎn)生，并可能反映了多能成體干細胞中Sox2、Kfl4和c-Myc的內(nèi)源性表達水平。

此外，由于Oct3/4啟動子具有眾所周知的響應元件和轉(zhuǎn)錄控制機制，通過使用小分子試劑選擇性激活Oct3/4的表達，基于環(huán)境線索的重新編程現(xiàn)在是可行的。我們研究的表達Oct3/4的人MSCs可以進一步研究iPSC機制。

優(yōu)化你的hMSC培養(yǎng):

1)在1 - 5% O2, 5% CO2中培養(yǎng)細胞，氣體含量以N2平衡。

2)強烈推薦使用MSCGroTM媒體。

3)減量-我們推薦Greiner Bio-one tc處理的細胞培養(yǎng)瓶或同等的。

動物種別/Organism  人

組織來源 Tissue and Cell Type 多能干細胞

形態(tài)/Morphology 貼壁生長 

注意事項：此產(chǎn)品僅供科研使用