背景介紹/context：

Mesenchymal Stem Progeitors are derived from human embryonic cell line, H9 (WA09). Cells are >80% CD90, CD105, CD166 and CD73 positive and<5% Oct4 positive. Cells have the capabilties to: 

Form adherent monolayers – ideal for 96- and 384-well formats for high throughput and high content cell-based assays.

To differentiate into a wide variety of mesenchymal subtypes, including osteogenic and chondrogenic lineages (but not adipogenic)– ideal for drug discovery for a wide variety of biological targets and basic research in bone, cartilage, metabolic and immunological diseases.

Passage up to 10X.

Note: Please include a 30 USD dry ice charge when ordering. Pricing is for US and Canada only.

Specifications: 

Cell Type: mesenchymal progenitor cells 

Species: human 

Source: derived from human embryonic cell line, H9 (WA09)

 Morphology: fibroblastic

Quantity: >1x10^6 cells per vial (viable post-thaw) 

Shipping method: dry ice

Storage: liquid nitrogen

Growth Properties: adherent

 proliferative Medium: αMEM Medium supplemented with 10% FBS, Pen/Strep and L-glutamine

 Phenotyping: Cells are >80% CD90, CD105, CD166 and CD73 positive and<5% Oct4 positive. DNA fingerprint: Results are consistent with the presence of a single cell line with no evidence of culture cross contamination. 

Pathogen testing: This lot was derived from the H9 hESC line that has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV. The H9 cell line has been tested and shown to be negative (performed by GIVF Laboratories). Mesenchymal cells are also tested for sterility and mycoplasma contamination.

間充質(zhì)干細(xì)胞祖細(xì)胞來(lái)源于人胚胎細(xì)胞系H9 (WA09)。細(xì)胞中CD90、CD105、CD166、CD73陽(yáng)性占80%，Oct4陽(yáng)性占5%。細(xì)胞具有以下能力:

形成貼壁單分子膜-理想的96孔和384孔格式的高通量和高含量的細(xì)胞為基礎(chǔ)的分析。
分化為多種間充質(zhì)亞型，包括成骨和軟骨源性譜系(但不包括脂肪源性)，是發(fā)現(xiàn)多種生物靶點(diǎn)和骨、軟骨、代謝和免疫疾病基礎(chǔ)研究的理想藥物。

傳代可達(dá)到10個(gè)倍增。

注:請(qǐng)?jiān)谟嗁?gòu)時(shí)包括30美元的干冰費(fèi)用。價(jià)格僅針對(duì)美國(guó)和加拿大。

規(guī)格:

細(xì)胞類型:間充質(zhì)祖細(xì)胞

種類:人

來(lái)源:來(lái)自人類胚胎細(xì)胞系,H9 (WA09)

形態(tài):成纖維細(xì)胞

數(shù)量:每瓶1x10^6細(xì)胞(解凍后活細(xì)胞)

運(yùn)輸方法:干冰

保存方法:液態(tài)氮增長(zhǎng)

屬性:貼壁生長(zhǎng) 

增殖培養(yǎng)基:補(bǔ)充10%胎牛血清、Pen/Strep和l -谷氨酰胺的αMEM培養(yǎng)基

表現(xiàn)型:細(xì)胞中CD90、CD105、CD166、CD73陽(yáng)性占80%，Oct4陽(yáng)性占5%。

DNA指紋圖譜:結(jié)果與單細(xì)胞的存在一致，沒(méi)有培養(yǎng)交叉污染的證據(jù)。

病原體檢測(cè):該批次來(lái)自H9 hESC細(xì)胞系，已對(duì)乙型肝炎、丙型肝炎、HIV-1、HIV-2、HTLV-I/II、HSV1、HSV2、EBV和CMV進(jìn)行檢測(cè)。H9細(xì)胞系已經(jīng)過(guò)測(cè)試，顯示為陰性(由GIVF實(shí)驗(yàn)室進(jìn)行)。還測(cè)試間充質(zhì)細(xì)胞的無(wú)菌性和支原體污染。

注意事項(xiàng)：此產(chǎn)品僅供科研使用