C57BL/6 Mouse Primary Embryonic Fibroblasts from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Embryonic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 2x10^6 cells per ml and is delivered frozen. Mouse Primary Embryonic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. Cells can be expanded for 3-6 passages at a split ratio of 1: 2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.

For research use only