Mouse iPS cell line (induced pluripotent stem cell line) was derived from mouse embryonic fibroblasts (MEFs) by retroviral expression of Oct3/4, Sox2, Klf4 and c-Myc genes. The cells were derived using morphological selection criteria and without the use of fluorescent marker or drug selection. When cultured under standard mouse ES cell culture conditions, the morphology of mouse iPSCs are identical to that of mouse ES cells. The cells also express the pluripotency markers SSEA-1 and Nanog, and demonstrate strong endogenous alkaline phosphatase activity. Mouse iPS cells are grown on a feeder layer of mouse embryonic fibroblasts (MEFs) and require the pretreatment of the plate with Gelatin.

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