背景介紹/context：

AcceGen Human iPSC-Derived Neural Stem Cells (NSCs) are derived from integration-free, induced pluripotent stem cells (iPSCs) under fully defined neural induction conditions. The NSCs express typical markers of cerebral cortical neural stem and progenitor cells such as PAX6, FOXG1 and nestin, and spontaneously form polarized neural tube-like rosette structures when plated as a monolayer in culture (see below). Additionally, AcceGen NSCs are capable of generating a spectrum of cerebral cortical excitatory and inhibitory neurons that are electrically active and have the ability to form functional synapses and circuits in vitro. After thawing and plating, the neural stem cells terminally differentiate to acquire mature electrophysiological properties, and form functional synaptic networks over a period of 40 ~ 50 days.

AcceGen NSCs are easy to differentiate to neurons or mixed neural cell types, following our protocols and using our tailored media and reagent bundles. A highly pure population of neurons can be generated from AcceGen NSCs following the synchronous differentiation protocol. Using our specialized coating reagents, neurons derived from AcceGen NSCs can be maintained in culture long-term (>1 year). NSCs are available from multiple donors to suit your research needs and have been characterized extensively.

AcceGen人ipsc來源的神經(jīng)干細(xì)胞(NSCs)是在完全確定的神經(jīng)誘導(dǎo)條件下由無整合誘導(dǎo)的多能干細(xì)胞(iPSCs)衍生而來。NSCs表達(dá)典型的大腦皮層神經(jīng)干和祖細(xì)胞標(biāo)志物，如PAX6、FOXG1和nestin，并在培養(yǎng)中作為單層被鍍時(shí)自發(fā)形成極化神經(jīng)管狀玫瑰叢結(jié)構(gòu)(見下)。此外，AcceGen NSCs還能產(chǎn)生一系列腦皮層興奮性和抑制性神經(jīng)元，這些神經(jīng)元具有電活性，并能在體外形成功能性突觸和回路。經(jīng)解凍和電鍍后，神經(jīng)干細(xì)胞在40 ~ 50天內(nèi)最終分化，獲得成熟的電生理特性，形成功能突觸網(wǎng)絡(luò)。

AcceGen NSCs很容易區(qū)分為神經(jīng)元或混合神經(jīng)細(xì)胞類型，遵循我們的協(xié)議，并使用我們定制的介質(zhì)和試劑束。根據(jù)同步分化協(xié)議，AcceGen NSCs可生成高度純的神經(jīng)元群。使用我們專門的包衣試劑，從AcceGen神經(jīng)干細(xì)胞中提取的神經(jīng)元可以在長(zhǎng)期培養(yǎng)中維持(1年)。NSCs可以從多個(gè)捐贈(zèng)者那里獲得，以滿足您的研究需要，并具有廣泛的特征。

動(dòng)物種別/Organism  人

組織來源 Tissue and Cell Type 神經(jīng)干細(xì)胞

形態(tài)/Morphology 貼壁生長(zhǎng)

注意事項(xiàng)：此產(chǎn)品僅供科研使用