背景介紹/context：

Trisomy X (Triple X) syndrome occurs at a frequency of approximately 1 in 1000 newborn females. The syndrome is associated with an increased risk of seizures and may affect short-term memory and learning although the majority of women affected show few clinical symptoms. AcceGen has developed iPSC-derived neural stem cells from a patient with the triple X chromosomal abnormality. All of the iPS cells examined (20 individual cells karyotyped), had trisomy X.AcceGen Human iPSC-Derived Neural Stem Cells (NSCs) are derived from integration-free, induced pluripotent stem cells (iPSCs) under fully defined neural induction conditions. The NSCs express typical markers of cerebral cortical neural stem and progenitor cells such as PAX6, FOXG1 and nestin, and spontaneously form polarized neural tube-like rosette structures when plated as a monolayer in culture (see below). Additionally, AcceGen NSCs are capable of generating a spectrum of cerebral cortical excitatory and inhibitory neurons that are electrically active and have the ability to form functional synapses and circuits in vitro. After thawing and plating, the neural stem cells terminally differentiate to acquire mature electrophysiological properties, and form functional synaptic networks over a period of 40 ~ 50 days.

AcceGen NSCs are easy to differentiate to neurons or mixed neural cell types, following our protocols and using our tailored media and reagent bundles. A highly pure population of neurons can be generated from AcceGen NSCs following the synchronous differentiation protocol. Using our specialized coating reagents, neurons derived from AcceGen NSCs can be maintained in culture long-term (>1 year). NSCs are available from multiple donors to suit your research needs and have been characterized extensively.

三染色體綜合征的發(fā)生率約為千分之一。該綜合征與癲癇發(fā)作風險增加有關(guān)，并可能影響短期記憶和學習，盡管大多數(shù)受影響的女性幾乎沒有臨床癥狀。AcceGen已經(jīng)從一名三倍X染色體異?；颊呱砩祥_發(fā)出ipsc來源的神經(jīng)干細胞。人類ipsc來源的神經(jīng)干細胞(NSCs)是在完全確定的神經(jīng)誘導條件下由無整合誘導的多能干細胞(iPSCs)產(chǎn)生的。NSCs表達典型的大腦皮層神經(jīng)干和祖細胞標志物，如PAX6、FOXG1和nestin，并在培養(yǎng)中作為單層被鍍時自發(fā)形成極化神經(jīng)管狀玫瑰叢結(jié)構(gòu)(見下)。此外，AcceGen NSCs還能產(chǎn)生一系列腦皮層興奮性和抑制性神經(jīng)元，這些神經(jīng)元具有電活性，并能在體外形成功能性突觸和回路。經(jīng)解凍和電鍍后，神經(jīng)干細胞在40 ~ 50天內(nèi)最終分化，獲得成熟的電生理特性，形成功能突觸網(wǎng)絡(luò)。

AcceGen NSCs很容易區(qū)分為神經(jīng)元或混合神經(jīng)細胞類型，遵循我們的協(xié)議，并使用我們定制的介質(zhì)和試劑束。根據(jù)同步分化協(xié)議，AcceGen NSCs可生成高度純的神經(jīng)元群。使用我們專門的包衣試劑，從AcceGen神經(jīng)干細胞中提取的神經(jīng)元可以在長期培養(yǎng)中維持(1年)。NSCs可以從多個捐贈者那里獲得，以滿足您的研究需要，并具有廣泛的特征。

動物種別/Organism  人

組織來源 Tissue and Cell Type 神經(jīng)干細胞(NSCs)

形態(tài)/Morphology 貼壁生長

注意事項：此產(chǎn)品僅供科研使用