C-33 A 細(xì)胞株是N. Auersperg從宮頸癌切片中建立的一系列細(xì)胞株(參見ATCC CRL-1594和ATCC CRL-1595)中的一株。取樣于66歲女性患者， 細(xì)胞一開始就表現(xiàn)出亞二倍體核型及上皮細(xì)胞形態(tài)。 連續(xù)傳代可以觀察到核型不穩(wěn)定。 存在成視網(wǎng)膜細(xì)胞瘤蛋白(pRB)，但大小不正常。 P53表達(dá)上調(diào)，且有一個(gè)273位密碼子的點(diǎn)突變導(dǎo)致Arg -> Cys的替換。 人乳頭瘤病毒DNA及RNA陰性。

組織來源/Tissue and Cell Type： 宮頸癌

形態(tài)/Morphology： 上皮細(xì)胞樣，貼壁生長(zhǎng)；

培養(yǎng)基和添加劑/Complete Growth Medium and Culture Conditions：

90%MEM(MD0300)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培養(yǎng)基：MEM完全培養(yǎng)基（貨號(hào)：MD0301）

氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

凍存條件   基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS

完全凍存液：免程序降溫細(xì)胞凍存液（低血清）(SD0001)

注意事項(xiàng)：此產(chǎn)品僅供科研使用。

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