BALB/c小鼠AcceGen原代肺泡上皮細(xì)胞從無致病性實驗小鼠組織中分離。小鼠原代肺泡上皮細(xì)胞在預(yù)涂明膠包衣液的T25組織培養(yǎng)瓶中生長0.5小時，在AcceGen完全生長培養(yǎng)基中培養(yǎng)3-7天。細(xì)胞從培養(yǎng)瓶中分離出來，立即冷凍保存在小瓶中。每瓶至少含有1x10^6細(xì)胞每毫升，并冷凍運送。小鼠原代肺泡上皮細(xì)胞可用于細(xì)胞間的粘附和遷移。上皮細(xì)胞培養(yǎng)的標(biāo)準(zhǔn)生化程序包括RT-PCR、Western blotting、免疫沉淀、免疫熒光染色或免疫熒光流式細(xì)胞術(shù)或用于產(chǎn)生所需研究應(yīng)用的細(xì)胞衍生物。

BALB/c Mouse Primary Alveolar Epithelial Cells from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Alveolar Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen's Culture Complete Growth Medium for 3-7 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Primary Alveolar Epithelial Cells can be used in assays of cell to cell adhesion and migration. Standard biochemical procedures performed with epithelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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