BALB/c小鼠AcceGen原代主動(dòng)脈成纖維細(xì)胞從無致病性實(shí)驗(yàn)小鼠組織中分離。小鼠原代主動(dòng)脈成纖維細(xì)胞在涂有明膠基溶液的T25組織培養(yǎng)瓶中生長0.5小時(shí)，一般在AcceGen培養(yǎng)完全生長培養(yǎng)基進(jìn)行3-7天的擴(kuò)增純化培養(yǎng)。裝運(yùn)前，細(xì)胞從瓶中分離出來，立即冷凍保存在小瓶中。每瓶至少含有1x10^6細(xì)胞每毫升，并冷凍運(yùn)送。小鼠主動(dòng)脈原代成纖維細(xì)胞對(duì)細(xì)菌、酵母、真菌和支原體呈陰性。免疫熒光染色檢測(cè)細(xì)胞標(biāo)記物的表達(dá)，抗體為anti-FSP1/S100A4。在AcceGen規(guī)定的細(xì)胞培養(yǎng)條件下，細(xì)胞可以1:2的分裂比擴(kuò)增2-4代。不建議多次傳代和保種。此細(xì)胞可用于細(xì)胞培養(yǎng)的標(biāo)準(zhǔn)生化程序包括測(cè)定細(xì)胞間相互作用、RT-PCR、Western blotting、免疫沉淀、免疫熒光染色、流式細(xì)胞術(shù)或產(chǎn)生用于所需研究應(yīng)用的細(xì)胞衍生物。

BALB/c Mouse Primary Aortic Fibroblasts from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Aortic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Primary Aortic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended. Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

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